Nutrigenomics and Fish Growth Endocrinology Group


Institute of aquaculture Torre de la Sal, CSIC
ANALYTICAL TOOLS

Array layout, Sea bream Mito-Chip

Array of selected markers of mitochondrial biogenesis and activity

PCR-Arrays

Bermejo-Nogales et al., 2014, General and Comparative Endocrinology 205; 305-315

Martos-Sitcha et al., 2017. Frontiers in Zoology 14:34



Array layout, Sea bream OXPHOS-Chip

A complete set of oxidative phosphorylation (88 genes)-mitochondrial respiratory chain, including mitochondrial- and nuclear-encoded genes of complexes I, II, III, IV and V, and their assembly proteins

PCR-Arrays

Bermejo-Nogales et al., 2015, PLOS One 10: e0122889

Martos-Sitcha et al., 2017. Frontiers in Zoology 14:34



Array layout, Sea bream Lipid-Chip

Array of 40 selected markers of lipid metabolism

PCR-Arrays

Benedito-Palos et al., 2014; Gene 547; 34-42



Array layout, Sea bream Interleukin-Chip

Array of 19 immunorelevant genes, including ILs and IL-receptors

PCR-Arrays

Pérez-Cordón et al., 2014, Fish & Shellfish Immunology 37; 201-208



Sea bream- Circadian gene regulation in whole larva

Customized Agilent oligoarray (8x15 k); 13939 annotated genes (sea_bream_nutrigroup_array v.3)

Microarrays

Yúfera et al., 2017. Scientific Reports 7:12943.



Array layout, Sea bream Oxidative Stress-chip

Array of 34 selected markers of oxidative stress

PCR-Arrays

Pérez-Sánchez et al., 2013 Comparative Biochemistry & Physiology 8D; 123-130

Magnoni et al., 2017. Biology Open 6, 897-908



Sea bass- Funtional specialization along the intestinal transcriptome

Customized Agilent oligoarray (8x15 k); 14147 different annotated genes (sea_bass_nutrigroup_array v.1)

Microarrays

Calduch-Giner et al., 2016. Frontiers in Physiology 7:359



Array layout, Sea bream Gut-chip

Array of 88 selected markers of intestinal function and integrity.

PCR-Arrays

Pérez-Sánchez et al., 2015. Fish & Shellfish Immunology 44, 117-128

Estensoro et al., 2016. PLoS ONE 11:e0166564

Gisbert et al., 2017. British Journal of Nutrition 117, 351-363



Sea bream- Tissue-specific response after hyper- and hypo-osmotic challenges

Customized Agilent oligoarray (4 x 40 k); 6862 annotarted sequences + 8294 non-annotated sequences (AQUAGENOMICS array)

Microarrays

Martos-Sitcha et al., 2016; PLoS ONE 11:e0148113



Muscle transcriptome/Meta-analysis of microarray gene expression profiling in fish

Customized Agilent oligoarray (4 x 40 k); 6862 annotarted sequences + 8294 non-annotated sequences (AQUAGENOMICS array)

Meta-analysis tool (Fish & Chips, AQUAEXCEL Poject)

Microarrays

Calduch-Giner et al., 2014; Marine Biotechnology 16:423–435



Sea bream- Nutritional regulation of intestinal transcriptome

Customized Agilent oligoarray (8x15 k); 7000 annotated genes genes (AQUAMAX array, sea_bream_nutrigroup_array v.2)

Microarrays

Calduch-Giner et al., 2012, BMC Genomics 13:470



Sea bass, Lipid-Chip

Array of 29 selected markers of lipid metabolism

PCR-Arrays

Rimoldi et al., 2016. Reviews in Fish Biology and Fisheries 26, 93-108



Sea bream- Gene expression profiling of head kidney and intestine in parasitized fish

18490 cDNA clones (AQUAFIRST array, sea_bream_nutrigroup_array v.1)

Microarrays

Davey et al., 2011, Molecular Immunology 48;2102-2112



Sea bream- Gene expression profiling of liver in crowded stressed fish

18490 cDNA clones (AQUAFIRST array, sea_bream_nutrigroup_array v.1)

Microarrays

Calduch-Giner et al., 2010, BMC Genomics 11:193



Sea bream, Growth-Chip

Array of 87 selected growth markers, including markers of GH/IGF system, muscle growth and cell differentiation and proliferation, protein folding, assembly and breakdown, and inflammatory or anti-inflammatory responses.

PCR-Arrays

Benedito-Palos et al., 2016. Aquaculture 454, 8-18




Administration