Nutrigenomics and Fish Growth Endocrinology Group


Institute of aquaculture Torre de la Sal, CSIC
ANALYTICAL TOOLS

Muscle transcriptome/Meta-analysis of microarray gene expression profiling in fish/

Customized Agilent oligoarray (4 x 40 k); 6862 annotarted sequences + 8294 non-annotated sequences (AQUAGENOMICS array)

Meta-analysis tool (Fish & Chips, AQUAEXCEL Poject)

Microarrays

Calduch-Giner et al., 2014; Marine Biotechnology 16:423–435



Sea bream- Nutritional regulation of intestinal transcriptome

Customized Agilent oligoarray (8x15 k); 7000 annotated genes genes (AQUAMAX array)

Microarrays

Calduch-Giner et al., 2012, BMC Genomics 13:470



Sea bream- Gene expression profiling of head kidney and intestine in parasitized fish

18490 cDNA clones (AQUAFIRST array)

Microarrays

Davey et al., 2011, Molecular Immunology 48;2102-2112



Sea bream- Gene expression profiling of liver in crowded stressed fish

18490 cDNA clones (AQUAFIRST array)

Microarrays

Calduch-Giner et al., 2010, BMC Genomics 11:193



Array layout, Sea bream Mito-Chip

Array of 60 selected markers of mitochondrial biogenesis and activity

PCR-Arrays

Bermejo-Nogales et al., 2014, General and Comparative Endocrinology 205; 305-315



Array layout, Sea bream OXPHOS-Chip

A complete set of oxidative phosphorylation (88 genes)-mitochondrial respiratory chain, including mitochondrial- and nuclear-encoded genes of complexes I, II, III, IV and V, and their assembly proteins

PCR-Arrays

Bermejo-Nogales et al., 2015, PLOS One 10: e0122889 (2015)



Array layout, Sea bream Lipid-Chip

Array of 40 selected markers of lipid metabolism

PCR-Arrays

Benedito-Palos et al., 2014; Gene 547; 34-42



Array layout, Sea bream Interleukin-Chip

Array of 19 immunorelevant genes, including ILs and IL-receptors

PCR-Arrays

Pérez-Cordón et al., 2014, Fish & Shellfish Immunology 37; 201-208



Array layout, Sea bream Oxidative Stress-chip

Array of 34 selected markers of oxidative stress

PCR-Arrays

Pérez-Sánchez et al., 2013 Comparative Biochemistry & Physiology 8D; 123-130



Array layout, Sea bream Gut-chip

Array of 88 selected markers of intestinal function and integrity.

PCR-Arrays

Pérez-Sánchez et al., 2015. Fish & Shellfish Immunology 44, 117-128




Administration