Array layout, Sea bream Mito-Chip
PCR-Arrays
PCR-array layout of sea bream mito-chip with extra-wells for housekeeping genes and controls of PCR performance.
Mitochondrial chaperones: mtHsp10, DnaJA3A, DnaJC20, mtHsp60, mtHsp70
Endoplasmic reticulum stress response: DER-1, ERdj, GRP-170
Antioxidant defense: CAT, GPX4, GR, GST3, PRDX3, PRDX5, SOD2
Oxidative metabolism: ACAA2, CPT1A, CPT1B, ECH, HADH, CS, IDH3A, IDH3B, IDH3G, Cox4a
Mitochondrial respiration uncoupling: UCP1, UCP2, UCP3
Outer membrane translocases (TOM complex): Tom70, Tom34, Tom22, Tom7, Tom5
Inner membrane translocases (TIM23 complex): Tim44, Tim23, Tim17A, Tim16, Tim14, Tim13, Tim8A
Inner membrane translocases (TIM22 complex): Tim22, Tim10, Tim9
Mitochondrial dynamics: FIS1, MIFFB, MFN1, MFN2, MIRO1A, MIRO2
Apoptosis: AIFM1, AIFM3, BAX, BCL2, BCLX
Nuclear transcription factors: mtTFA, NRF1, NRF2, PGC1α, PGC1β
Housekeeping genes: ACTB, EF-1, α-tubulin, 18S rRNA
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1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
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A |
Hsp10 |
CAT |
ACAA2 |
IDH3B |
Tom22 |
Tim13 |
MIFFB |
PERP |
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PPC1 |
PPC1 |
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B |
DnaJA3A |
GPX4 |
CPT1A |
IDH3G |
Tom7 |
Tim8A |
MIRO1A |
GABPA |
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PPC2 |
PPC2 |
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C |
DnaJC20 |
GR |
CPT1B |
COX4a |
Tom5 |
Tim22 |
MIRO2 |
mtTFA |
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PPC3 |
PPC3 |
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D |
Hsp60 |
GST3 |
ECH |
UCP1 |
Tim44 |
Tim10 |
AIFM1 |
NRF1 |
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PPC4 |
PPC4 |
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E |
Grp-75 |
PRDX3 |
HADH |
UCP2 |
Tim23 |
Tim9 |
AIFM3 |
PGC1α |
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NPC |
NPC |
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F |
DER-1 |
PRDX5 |
CS |
UCP3 |
Tim17A |
FIS1 |
BAX |
PGC1β |
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ACTB |
ACTB |
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G |
ERdj3 |
SOD1 |
IDH1 |
Tom70 |
Tim16 |
MFN1 |
BCL2 |
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H |
Grp170 |
SOD2 |
IDH3A |
Tom34 |
Tim14 |
MFN2 |
BCLX |
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