Nutrigenomics and Fish Growth Endocrinology Group


Institute of aquaculture Torre de la Sal, CSIC


Array layout, Sea bream Mito-Chip
PCR-Arrays

PCR-array layout of sea bream mito-chip with extra-wells for housekeeping genes and controls of PCR performance.

 Mitochondrial chaperones: mtHsp10, DnaJA3A, DnaJC20, mtHsp60, mtHsp70

Endoplasmic reticulum stress response: DER-1, ERdj, GRP-170

Antioxidant defense: CAT, GPX4, GR, GST3, PRDX3, PRDX5, SOD2

Oxidative metabolism: ACAA2, CPT1A, CPT1B, ECH, HADH, CS, IDH3A, IDH3B, IDH3G, Cox4a

Mitochondrial respiration uncoupling: UCP1, UCP2, UCP3

Outer membrane translocases (TOM complex): Tom70, Tom34, Tom22, Tom7, Tom5

Inner membrane translocases (TIM23 complex): Tim44, Tim23, Tim17A, Tim16, Tim14, Tim13, Tim8A

Inner membrane translocases (TIM22 complex): Tim22, Tim10, Tim9

Mitochondrial dynamics: FIS1, MIFFB, MFN1, MFN2, MIRO1A, MIRO2

Apoptosis: AIFM1, AIFM3, BAX, BCL2, BCLX

Nuclear transcription factors: mtTFA, NRF1, NRF2, PGC1α, PGC1β 

Housekeeping genes: ACTB, EF-1, α-tubulin, 18S rRNA

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

Hsp10

CAT

ACAA2

IDH3B

Tom22

Tim13

MIFFB

PERP

 

 

PPC1

PPC1

B

DnaJA3A

GPX4

CPT1A

IDH3G

Tom7

Tim8A

MIRO1A

GABPA

 

 

PPC2

PPC2

C

DnaJC20

GR

CPT1B

COX4a

Tom5

Tim22

MIRO2

mtTFA

 

 

PPC3

PPC3

D

Hsp60

GST3

ECH

UCP1

Tim44

Tim10

AIFM1

NRF1

 

 

PPC4

PPC4

E

Grp-75

PRDX3

HADH

UCP2

Tim23

Tim9

AIFM3

PGC1α

 

 

NPC

NPC

F

DER-1

PRDX5

CS

UCP3

Tim17A

FIS1

BAX

PGC1β

 

 

ACTB

ACTB

G

ERdj3

SOD1

IDH1

Tom70

Tim16

MFN1

BCL2

 

 

 

 

 

H

Grp170

SOD2

IDH3A

Tom34

Tim14

MFN2

BCLX

 

 

 

 

 




Administration