Array layout, Sea bream Oxidative Stress-chip
PCR-Arrays
PCR-array layout of oxidative stress-chip (34 genes x 2 samples per plate) with extra-wells for housekeeping genes and general controls of PCR performance.
Energy metabolism: Cox4a, CPT1A, ECH, HADH, H-FABP, HL, HSL, LAL, LPL, LPL-like, UCP1.
Growth factors: IGF-I, IGF-II.
Antioxidant enzymes: CAT, Cu-Zn-SOD, GPX1, GPX4, GR, HEPC, Mn-SOD, PRDX3, PRDX5.
Molecular chaperons and RE stress response: DER-1, ERj3, GRP-170, GRP- 94, GRP-75.
Xenobiotic metabolism: AHR1, CYP1A1, GST3.
Nuclear receptors: ER-α, GCR.
Transcription factors: CREB-2, HIF-1α.
Housekeeping: ACTB
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
GRP-170 |
CYP1A1 |
GPX4 |
IGF-I |
Mn-SOD |
GRP-170 |
CYP1A1 |
GPX4 |
IGF-I |
Mn-SOD |
PPC1 |
PPC1 |
|
B |
GRP-94 |
DER-1 |
GR |
IGF-II |
UCP1 |
GRP-94 |
DER-1 |
GR |
IGF-II |
UCP1 |
PPC2 |
PPC2 |
|
C |
GRP-75 |
ECH |
GST3 |
LPL |
|
GRP-75 |
ECH |
GST3 |
LPL |
|
PPC3 |
PPC3 |
|
D |
AHR1 |
ERj3 |
HL |
LPL-like |
|
AHR1 |
ERj3 |
HL |
LPL-like |
|
PPC4 |
PPC4 |
|
E |
CPT1A |
ER-α |
HEPC |
LAL |
|
CPT1A |
ER-α |
HEPC |
LAL |
|
ACTB |
ACTB |
|
F |
CAT |
H-FABP |
HSL |
PRDX3 |
|
CAT |
H-FABP |
HSL |
PRDX3 |
|
ACTB |
ACTB |
|
G |
CREB-2 |
GCR |
HADH |
PRDX5 |
|
CREB-2 |
GCR |
HADH |
PRDX5 |
|
NPC |
NPC |
|
H |
Cox4a |
GPX1 |
HIF-1α |
Cu-Zn-SOD |
|
Cox4a |
GPX1 |
HIF-1α |
Cu-Zn-SOD |
|
NPC |
NPC |
