Nutrigenomics and Fish Growth Endocrinology Group


Institute of aquaculture Torre de la Sal, CSIC


Array layout, Sea bream Oxidative Stress-chip
PCR-Arrays

PCR-array layout of oxidative stress-chip (34 genes x 2 samples per plate) with extra-wells for housekeeping genes and general controls of PCR performance.

Energy metabolism: Cox4a, CPT1A, ECH, HADH, H-FABP, HL, HSL, LAL, LPL, LPL-like, UCP1.

Growth factors: IGF-I, IGF-II.

Antioxidant enzymes: CAT, Cu-Zn-SOD, GPX1, GPX4, GR, HEPC, Mn-SOD, PRDX3, PRDX5.

Molecular chaperons and RE stress response: DER-1, ERj3, GRP-170, GRP- 94, GRP-75.

Xenobiotic metabolism: AHR1, CYP1A1, GST3.

Nuclear receptors: ER-α, GCR.

Transcription factors: CREB-2, HIF-1α.

Housekeeping: ACTB

 

1

2

3

4

5

6

7

8

9

10

11

12

A

GRP-170

CYP1A1

GPX4

IGF-I

Mn-SOD

GRP-170

CYP1A1

GPX4

IGF-I

Mn-SOD

PPC1

PPC1

B

GRP-94

DER-1

GR

IGF-II

UCP1

GRP-94

DER-1

GR

IGF-II

UCP1

PPC2

PPC2

C

GRP-75

ECH

GST3

LPL

 

GRP-75

ECH

GST3

LPL

 

PPC3

PPC3

D

AHR1

ERj3

HL

LPL-like

 

AHR1

ERj3

HL

LPL-like

 

PPC4

PPC4

E

CPT1A

ER-α

HEPC

LAL

 

CPT1A

ER-α

HEPC

LAL

 

ACTB

ACTB

F

CAT

H-FABP

HSL

PRDX3

 

CAT

H-FABP

HSL

PRDX3

 

ACTB

ACTB

G

CREB-2

GCR

HADH

PRDX5

 

CREB-2

GCR

HADH

PRDX5

 

NPC

NPC

H

Cox4a

GPX1

HIF-1α

Cu-Zn-SOD

 

Cox4a

GPX1

HIF-1α

Cu-Zn-SOD

 

NPC

NPC




Administration