Array layout, Sea bream Interleukin-Chip
PCR-Arrays

PCR-array layout of IL-chip (19 gens x 4 samples per plate) with extra-wells for housekeeping genes and general controls of PCR performance

IL-I family and related receptors: IL-1b, IL-1R1, IL-1R2, IL-18, IL-18R1.
IL-6 type family and related receptors: IL-6, IL-6RA, IL-6RB.
Common g-chain cytokine family: IL-7, IL-15.
CXC chemokine family and related receptors: IL-8, IL8-RA.
IL-10 family and related receptors: IL-10, IL-10RA, IL10RB, IL-22R.
IL-12 family: IL12b.
Other cytokines: IL34, TNF-a.

Housekeeping: ACTB

 

1

2

3

4

5

6

7

8

9

10

11

12

A

IL-1β

IL-7

IL-12β

IL-1β

IL-7

IL-12β

IL-1β

IL-7

IL-12β

IL-1β

IL-7

IL-12β

B

IL-1R1

IL-15

TNF-α

IL-1R1

IL-15

TNF-α

IL-1R1

IL-15

TNF-α

IL-1R1

IL-15

TNF-α

C

IL-1R2

IL-8

IL-34

IL-1R2

IL-8

IL-34

IL-1R2

IL-8

IL-34

IL-1R2

IL-8

IL-34

D

IL-18

IL-8RA

NPC

IL-18

IL-8RA

NPC

IL-18

IL-8RA

NPC

IL-18

IL-8RA

NPC

E

IL-18R1

IL-10

ACTB

IL-18R1

IL-10

ACTB

IL-18R1

IL-10

ACTB

IL-18R1

IL-10

ACTB

F

IL-6

IL-10RA

ACTB

IL-6

IL-10RA

ACTB

IL-6

IL-10RA

ACTB

IL-6

IL-10RA

ACTB

G

IL-6RA

IL-10RB

PPC1

IL-6RA

IL-10RB

PPC2

IL-6RA

IL-10RB

PPC3

IL-6RA

IL-10RB

ACTB

H

IL-6RB

IL-22R

PPC1

IL-6RB

IL-22R

PPC2

IL-6RB

IL-22R

PPC3

IL-6RB

IL-22R

PPC4




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