Nutrigenomics and Fish Growth Endocrinology Group


Institute of aquaculture Torre de la Sal, CSIC


Array layout, Sea bream Lipid-Chip
PCR-Arrays

PCR-array layout of lipid-chip (40 genes x 2 samples per plate) with extra-wells for housekeeping genes and general controls of PCR performance.

Elongases: ELOVL1b, ELOVL4, ELOVL5, ELOVL6, ELOVL7.

Desaturases: FADS2, SCD1a, SCD1b.

Phospholipid synthesis and remodeling enzymes: LPCAT1, LPCAT2, LPCAT3, PEMT, PLA2G12A, PLA2G12B, PLA2G15, RARRES3, PLA2G6, cyt-PLA2, int-PLA2.

Lipases and related genes: HL, LPL, LPL-like, LMF1, LIPH, LIPA, HSL, ATGL, DAGLB, ABHD6a, ABHD12b, CEL, cd-PL.

Fatty acid β-oxidation: CPT1A, CPT1B, ECH, HADH.

Transcription factors: LXRα, PPARα, PPARβ, PPARγ.

Housekeeping genes: ACTB

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ELOVL1b

LPCAT1

PLA2G6

LIPA

CPT1A

ELOVL1b

LPCAT1

PLA2G6

LIPA

CPT1A

PPC1

PPC1

B

ELOVL4

LPCAT2

cytPLA2

HSL

CPT1B

ELOVL4

LPCAT2

cytPLA2

HSL

CPT1B

PPC2

PPC2

C

ELOVL5

LPCAT3

intPLA2

ATGL

ECH

ELOVL5

LPCAT3

intPLA2

ATGL

ECH

PPC3

PPC3

D

ELOVL6

PEMT

HL

DAGLB

HADH

ELOVL6

PEMT

HL

DAGLB

HADH

PPC4

PPC4

E

ELOVL7

PLA2G12A

LPL

ABHD6a

LXRα

ELOVL7

PLA2G12A

LPL

ABHD6a

LXRα

NPC

NPC

F

FADS2

PLA2G12B

LPL-like

ABHD12b

PPARα

FADS2

PLA2G12B

LPL-like

ABHD12b

PPARα

NPC

NPC

G

SCD1a

PLA2G15

LMF1

CEL

PPARβ

SCD1a

PLA2G15

LMF1

CEL

PPARβ

ACTB

ACTB

H

SCD1b

RARRES3

LIPH

cd-PL

PPARγ

SCD1b

RARRES3

LIPH

cd-PL

PPARγ

ACTB

ACTB




Administration