Array layout, Sea bream Lipid-Chip
PCR-Arrays
PCR-array layout of lipid-chip (40 genes x 2 samples per plate) with extra-wells for housekeeping genes and general controls of PCR performance.
Elongases: ELOVL1b, ELOVL4, ELOVL5, ELOVL6, ELOVL7.
Desaturases: FADS2, SCD1a, SCD1b.
Phospholipid synthesis and remodeling enzymes: LPCAT1, LPCAT2, LPCAT3, PEMT, PLA2G12A, PLA2G12B, PLA2G15, RARRES3, PLA2G6, cyt-PLA2, int-PLA2.
Lipases and related genes: HL, LPL, LPL-like, LMF1, LIPH, LIPA, HSL, ATGL, DAGLB, ABHD6a, ABHD12b, CEL, cd-PL.
Fatty acid β-oxidation: CPT1A, CPT1B, ECH, HADH.
Transcription factors: LXRα, PPARα, PPARβ, PPARγ.
Housekeeping genes: ACTB
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ELOVL1b |
LPCAT1 |
PLA2G6 |
LIPA |
CPT1A |
ELOVL1b |
LPCAT1 |
PLA2G6 |
LIPA |
CPT1A |
PPC1 |
PPC1 |
|
B |
ELOVL4 |
LPCAT2 |
cytPLA2 |
HSL |
CPT1B |
ELOVL4 |
LPCAT2 |
cytPLA2 |
HSL |
CPT1B |
PPC2 |
PPC2 |
|
C |
ELOVL5 |
LPCAT3 |
intPLA2 |
ATGL |
ECH |
ELOVL5 |
LPCAT3 |
intPLA2 |
ATGL |
ECH |
PPC3 |
PPC3 |
|
D |
ELOVL6 |
PEMT |
HL |
DAGLB |
HADH |
ELOVL6 |
PEMT |
HL |
DAGLB |
HADH |
PPC4 |
PPC4 |
|
E |
ELOVL7 |
PLA2G12A |
LPL |
ABHD6a |
LXRα |
ELOVL7 |
PLA2G12A |
LPL |
ABHD6a |
LXRα |
NPC |
NPC |
|
F |
FADS2 |
PLA2G12B |
LPL-like |
ABHD12b |
PPARα |
FADS2 |
PLA2G12B |
LPL-like |
ABHD12b |
PPARα |
NPC |
NPC |
|
G |
SCD1a |
PLA2G15 |
LMF1 |
CEL |
PPARβ |
SCD1a |
PLA2G15 |
LMF1 |
CEL |
PPARβ |
ACTB |
ACTB |
|
H |
SCD1b |
RARRES3 |
LIPH |
cd-PL |
PPARγ |
SCD1b |
RARRES3 |
LIPH |
cd-PL |
PPARγ |
ACTB |
ACTB |
