Array layout, Sea bream Gut-chip
PCR-Arrays
PCR-array layout of 88 genes selected as markers of intestinal function and integrity with extra-wells for housekeeping genes and general controls of PCR performance.
Cell differentiation and proliferation: PCNA, BMP pathway (BMPR1A), Hh pathway (IHH, GLI1), Wnt pathway (HHIP, WLs, Myc, CTNNB1, Tcf4), Notch pathway (NLE1, HES1-B, GFI-1, KLF4).
Intestinal architecture and permeability: ITGB1BP1, ITGB6, ILK, OCLN, CLDN3, CLDN12, CLDN15, TJP1, CDH1, CDH17, F11R, CXADR, DSP, CX32.2, GJB4, MUC2, MUC2-like, MUC13, I-MUC.
Enterocyte mass and epithelia damage: ALPI, FABP1, FABP2, FABP6, CALR, CANX, GR, GST3, SOD1.
Interleukins and cytokines: IL1β, IL1R1, IL6, IL6RB, IL7, IL8, IL8RA, IL10, IL10RA, IL12B, IL34, TNFα, CTRP3, CSF1R1, CXCL9, CCL21, CCR3, CCR9, CR11, CCL20, CD48, CD276.
Pathogen recognition receptors: TLR1, TLR2, TLR5, TLR9, NOD1, MRC1, CD209, CD302, CLEC10A, LGALS1, LGALS8, CSL2, FCL, VIM.
Mitochondria function and biogenesis: mtHsp10, mtHsp60, mtHsp70, ECH, HADH, CS, Tim44, Tim22, mtTFA, NRF1, PGC1α.
Housekeeping: ACTB.
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
PCNA |
Tcf4 |
OCLN |
CXADR |
ALPI |
SOD1 |
IL8 |
CXCL9 |
TLR1 |
CLEC10A |
mtHsp70 |
PPC1 |
|
B |
BMPR1A |
NLE1 |
CLDN3 |
DSP |
FABP1 |
PRDX1 |
IL8RA |
CCL21 |
TLR2 |
LGALS1 |
ECH |
PPC2 |
|
C |
IHH |
HES1-B |
CLDN12 |
CX32.2 |
FABP2 |
PRDX2 |
IL10 |
CCR3 |
TLR5 |
LGALS8 |
HADH |
PPC3 |
|
D |
GLI1 |
GFI-1 |
CLDN15 |
GJB4 |
FABP6 |
IL1β |
IL10RA |
CCR9 |
TLR9 |
CSL2 |
CS |
PPC4 |
|
E |
HHIP |
KLF4 |
TJP1 |
MUC2 |
CALR |
IL1R1 |
IL12B |
CCR11 |
NOD1 |
FCL |
Tim44 |
NPC |
|
F |
WLs |
ITGB1BP1 |
CDH1 |
MUC2-like |
CANX |
IL6 |
TNFα |
CCL20 |
MRC1 |
VIM |
Tom22 |
ACTB |
|
G |
Myc |
ITGB6 |
CDH17 |
MUC13 |
GR |
IL6RB |
CTRP3 |
CD48 |
CD209 |
mtHsp10 |
mtTFA |
ACTB |
|
H |
CTNNB1 |
ILK |
F11R |
I-MUC |
GST3 |
IL7 |
CSF1R1 |
CD276 |
CD302 |
mtHsp60 |
NRF1 |
PGC1α |
