Nutrigenomics and Fish Growth Endocrinology Group


Institute of aquaculture Torre de la Sal, CSIC


Array layout, Sea bream Gut-chip
PCR-Arrays

PCR-array layout of 88 genes selected as markers of intestinal function and integrity with extra-wells for housekeeping genes and general controls of PCR performance.

Cell differentiation and proliferation: PCNA, BMP pathway (BMPR1A), Hh pathway (IHH, GLI1), Wnt pathway (HHIP, WLs, Myc, CTNNB1, Tcf4), Notch pathway (NLE1, HES1-B, GFI-1, KLF4).

Intestinal architecture and permeability: ITGB1BP1, ITGB6, ILK, OCLN, CLDN3, CLDN12, CLDN15, TJP1, CDH1, CDH17, F11R, CXADR, DSP, CX32.2, GJB4, MUC2, MUC2-like, MUC13, I-MUC.

Enterocyte mass and epithelia damage: ALPI, FABP1, FABP2, FABP6, CALR, CANX, GR, GST3, SOD1.

Interleukins and cytokines: IL1β, IL1R1, IL6, IL6RB, IL7, IL8, IL8RA, IL10, IL10RA, IL12B, IL34, TNFα, CTRP3, CSF1R1, CXCL9, CCL21, CCR3, CCR9, CR11, CCL20, CD48, CD276.

Pathogen recognition receptors: TLR1, TLR2, TLR5, TLR9, NOD1, MRC1, CD209, CD302, CLEC10A, LGALS1, LGALS8, CSL2, FCL, VIM.

Mitochondria function and biogenesis: mtHsp10, mtHsp60, mtHsp70, ECH, HADH, CS, Tim44, Tim22, mtTFA, NRF1, PGC1α.

Housekeeping: ACTB.

 

1

2

3

4

5

6

7

8

9

10

11

12

A

PCNA

Tcf4

OCLN

CXADR

ALPI

SOD1

IL8

CXCL9

TLR1

CLEC10A

mtHsp70

PPC1

B

BMPR1A

NLE1

CLDN3

DSP

FABP1

PRDX1

IL8RA

CCL21

TLR2

LGALS1

ECH

PPC2

C

IHH

HES1-B

CLDN12

CX32.2

FABP2

PRDX2

IL10

CCR3

TLR5

LGALS8

HADH

PPC3

D

GLI1

GFI-1

CLDN15

GJB4

FABP6

IL1β

IL10RA

CCR9

TLR9

CSL2

CS

PPC4

E

HHIP

KLF4

TJP1

MUC2

CALR

IL1R1

IL12B

CCR11

NOD1

FCL

Tim44

NPC

F

WLs

ITGB1BP1

CDH1

MUC2-like

CANX

IL6

TNFα

CCL20

MRC1

VIM

Tom22

ACTB

G

Myc

ITGB6

CDH17

MUC13

GR

IL6RB

CTRP3

CD48

CD209

mtHsp10

mtTFA

ACTB

H

CTNNB1

ILK

F11R

I-MUC

GST3

IL7

CSF1R1

CD276

CD302

mtHsp60

NRF1

PGC1α




Administration